The biggest barrier to reducing toxic pesticides in cannabis is, not surprisingly, the cannabis industry itself. Subscribe to NutritionFacts.org’s free newsletter to receive our B12 infographic that covers the latest research takeaways and Dr. Greger’s updated recommendations: https://nutritionfacts.org/subscribe/ For more on the link between the tobacco and marijuana industries, check out: Will Cannabis Turn Into Big Tobacco? (http://nutritionfacts.org/video/will-cannabis-turn-into-big-tobacco) I have a whole catalogue of marijuana videos and I’ve got more to come. Here are the ones that are already up in case you missed any: • The Institute of Medicine Report on the Health Effects of Marijuana (http://nutritionfacts.org/video/The-Institute-of-Medicine-Report-on-the-Health-Effects-of-Marijuana) • Researching the Health Effects of Marijuana (http://nutritionfacts.org/video/Researching-the-Health-Effects-of-Marijuana) • Is Marijuana Addictive? (http://nutritionfacts.org/video/Is-Marijuana-Addictive) • Does Marijuana Cause Health Problems? (http://nutritionfacts.org/video/Does-Marijuana-Cause-Health-Problems) • Does Marijuana Cause Permanent Brain Damage in Teens? (http://nutritionfacts.org/video/Does-Marijuana-Cause-Permanent-Brain-Damage-in-Teens) • Does Marijuana Cause Permanent Brain Damage in Adults? (http://nutritionfacts.org/video/Does-Marijuana-Cause-Permanent-Brain-Damage-in-Adults) • Marijuana Legalization and the Opioid Epidemic (http://nutritionfacts.org/video/Marijuana-Legalization-and-the-Opioid-Epidemic) • Effects of Smoking Marijuana on the Lungs (http://nutritionfacts.org/video/effects-of-smoking-marijuana-on-the-lungs) • Smoking Marijuana vs. Using a Cannabis Vaporizer (http://nutritionfacts.org/video/Smoking-Marijuana-vs-Using-a-Cannabis-Vaporizer) • Does Marijuana Cause Lung Cancer? (http://nutritionfacts.org/video/does-marijuana-cause-lung-cancer) • Can Cannabis Cure Cancer? (http://nutritionfacts.org/video/can-cannabis-cure-cancer) • Are Cannabis Edibles Safe? (http://nutritionfacts.org/video/are-cannabis-edibles-safe) More tobacco industry parallels can be found in Big Food Using the Tobacco Industry Playbook (http://nutritionfacts.org/video/big-food-using-the-tobacco-industry-playbook), American Medical Association Complicity with Big Tobacco (https://nutritionfacts.org/video/american-medical-association-complicity-big-tobacco/), and How Smoking in 1959 is Like Eating in 2019 (http://nutritionfacts.org/video/how-smoking-in-the-50s-is-like-eating-today). Have a question about this video? Leave it in the comment section at http://nutritionfacts.org/video/pesticides-in-marijuana and someone on the NutritionFacts.org team will try to answer it. Want to get a list of links to all the scientific sources used in this video? Click on Sources Cited at http://nutritionfacts.org/video/pesticides-in-marijuana. You’ll also find a transcript and acknowledgments for the video, my blog and speaking tour schedule, and an easy way to search (by translated language even) through our videos spanning more than 2,000 health topics. If you’d rather watch these videos on YouTube, subscribe to my YouTube Channel here: https://www.youtube.com/subscription_center?add_user=nutritionfactsorg Thanks for watching. I hope you’ll join in the evidence-based nutrition revolution! -Michael Greger, MD FACLM Captions for this video are available in several languages. To find yours, click on the settings wheel on the lower-right of the video and then "Subtitles/CC." Do you have feedback about the translations in this video? Please share it here along with the title of the video and language: https://nutritionfacts.zendesk.com/hc/requests/new To view the subtitles in transcript format, click on the ellipsis button below the video, choose "Open transcript", and select the language you'd like to view them in. Image credit: Andrea / Adobe Stock photos http://www.NutritionFacts.org • Subscribe: http://www.NutritionFacts.org/subscribe • Donate: http://www.NutritionFacts.org/donate • HOW NOT TO DIE: http://nutritionfacts.org/book • Facebook: http://www.facebook.com/NutritionFacts.org • Twitter: http://www.twitter.com/nutrition_facts • Instagram: http://instagram.com/nutrition_facts_org/ • Podcast : http://nutritionfacts.org/audio/

Scientia Pharmaceutica, an international, peer-reviewed Open Access journal.

Cervical cancer remains a global health related issue among females of Sub-Saharan Africa, with over half a million new cases reported each year. Different therapeutic regimens have been suggested in various regions of Africa, however, over a quarter of a million women die of cervical cancer, annually. This makes it the most lethal cancer amongst black women in this area, and makes it important to search for new effective therapeutic drugs through screening of medicinal plant extracts used by many in Sub-Saharan Africa as potential anti-cervical cancer agents. The aim of this study was to evaluate the anti-proliferative effects of Cannabis sativa extracts and its isolate, cannabidiol on cervical cancer cell lines HeLa, SiHa, and ME-180. To achieve our aim, phytochemical screening, MTT assay, cell growth analysis, flow cytometry, morphology analysis, Western blot, caspase 3/7 assay, and ATP measurement assay were conducted were conducted. Results obtained indicate that both plant extracts induced cell death at an IC50 of 50 – 100μg/ml and the Inhibition of cell growth was cell line dependent. Flow cytometry confirmed that, with or without cell cycle arrest, the type of induced cell death was apoptosis. Cannabis sativa extracts led to the up-regulation of apoptosis proteins (p53, Bax, caspase-3, and caspase-9) and the down regulation of anti-apoptosis proteins (Bcl-2 and RBBP6), signalling the execution of apoptosis. Apoptosis induction was further confirmed by morphological changes, an increase in Caspase 3/7 and a decrease in the ATP levels. In conclusion, this data implies Cannabis sativa crude extracts has the potential to inhibit growth and induce apoptosis in cervical cancer cell lines, which may be due to the presence of cannabidiol. Key words: Apoptosis, cervical cancer cells, cannabidiol, and Cannabis sativa extracts