Toll-like receptor 9 is involved in the induction of galectin-9 protein by dietary anti-allergic compound fucoidan
Dietary intervention of fucoidan extracted from Saccharina japonica brown seaweed has been ascertained to favor an increase of galectin-9 protein in the intestine of allergic mice, resulting in the attenuation of the food allergy symptoms. The molecular mechanism underpinning that galectin-9 secretion remains unclear. Recently, some evidence has suggested an implication of Toll-like receptor 9 (TLR9) in galectin-9 secretion. However, no investigation has been done. For this study, we aimed to understand the relationship between galectin-9 production and fucoidan intake, which will improve the therapeutic use of fucoidan in allergy treatment. Intestinal epithelial cells (IECs) were cultured in solid or transwell plates and apically exposed to fucoidan solutions and/or synthetic TLR9 agonist (CpG-ODN). The transcriptional response of the cells to galectin-9 (lgals9) and the TLR9 gene was evaluated by using q-RTPCR, and the protein expression of galectin-9 was analyzed by conducting an ELISA test. Knockdown of TLR9 in IECs was performed by targeting TLR9 siRNA, and its effect on galectin-9 release was assessed. We found that the interaction of fucoidan and IECs resulted in the upregulation of galectin-9 released in a dose- and time-dependent manner. The increase was further potentiated in combination with the TLR9 agonist. Fucoidan exposure to IECs tended to increase the mRNA expression of TLR9 in a way similar to that of the TLR9 agonist effect, and knockdown of TLR9 in IECs resulted in a decreased tendency of fucoidan-induced galectin-9 protein. TLR9 activation is therefore involved in the increased release of galectin-9 protein observed in IECs upon fucoidan exposure.
Ben Swann & Independent Media Announce Historic Lawsuit Led By RFK Jr. Against Media Giants - sovren.media
Robert F. Kennedy Jr. has just announced the filing of a groundbreaking lawsuit targeting Trusted News Initiative (TNI), comprised of media corporations includi...
Bone Health and Osteoporosis An Orthomolecular Perspective
Osteoporosis, like most other disease, is caused by many factors including deficiencies of essential nutrients such as vitamin D. But are prescription drugs really the answer?
It's Not Just Damar Hamlin, Hundreds of Athletes Globally Are Suddenly Collapsing From Cardiac Arrest - Videos - sovren.media
I first reported in November of 2021 about the sudden and new phenomenon of athletes collapsing from cardiac arrest on the field of play. In light of the collapse of Damar Hamlin's collapse during Monday Night Football on live television, it is vitally important that we ask the question "Why are so many athletes collapsing from cardiac arrest?"
These 11 Natural Compounds Help Build New Brain Cells GreenMedInfo
Your brain is capable of making new cells, but this capability declines with age. That is, unless you take action to combat it using lifestyle strategies and neuroprotective compounds found in nature
Myocarditis Once Rare, Now Common GreenMedInfo Blog Entry
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1 Know Labs Introduces KnowU, A Portable Medical-Grade Glucose Monitoring Device That Utilizes Bio-RFID Know Labs introduces KnowU, A Portable Glucose Monitoring Device
Know Labs, an emerging leader in non-invasive medical diagnostics, introduced the KnowU™, a new portable medical-grade glucose monitoring device that utilizes Bio-RFID™, Know Labs’ non-invasive diagnostic technology. Related Supersapiens Glucose Display Wearable Is Designed Specifically For Athletes The KnowU is a convenient, non-wearable, on-the-go alternative to fingersticks, which are used to check blood glucose levels, Know Labs introduced KnowU, a new portable medical-grade glucose monitoring device that utilizes Bio-RFID.
The effects of oral trehalose in patients with diabetes a pilot randomized controlled trial
Introduction: Trehalose is a naturally occurring disaccharide of two glucose molecules that has been suggested as a potential therapeutic agent to reduce blood glucose and ameliorate diabetes-related complications in type 2 diabetes (T2D). This study aimed to determine the efficacy of...
Metaformin Users - Consider This - David Perlmutter M.D.
Metformin is one of the most commonly prescribed medications on the planet. It is considered a “first-line” therapy for lowering blood glucose in the treatment of type-2 diabetes and has …
Inhibitory effects of fucoidan from Laminaria japonica against some pathogenic bacteria and SARS-CoV-2 depend on its large molecular weight - ScienceDirect
Fucoidan is a highly sulfated polysaccharide with a wide range of bioactivities, including anti-pathogenic activity. However, the relationship between…
Evidence of the Conspiracy To Commit Mass Murder by the DOD, HHS – Sasha Latypova - LewRockwell
In this presentation, Sasha Latypova covers the pseudo-legal structure, the organizational structure and the money flow of the COVID criminal enterprise. Latypova and I both highly recommend that you subscribe to Katherine Watt’s Bailiwick News Substack. This is the Transcript of Latypova’s conversation with Katherine Watt on November 4, 2022. Here are the DOD contracts for “COVID Countermeasures”. *** NOTES The topic of my presentation today is “Intent to Harm” and I’m going to discuss evidence of conspiracy – which is not a theory – of US Department of Defense, Health and Human Services, other government agencies and other governments all over the world, … Continue reading →
In this minireview, we examine the ability of modified citrus pectin (MCP), a complex water soluble indigestible polysaccharide obtained from the peel and pulp of citrus fruits and modified by means of high pH and temperature treatment, to affect numerous rate-limiting steps in cancer metastasis. Th …
Dr Isaac Eliaz's Modified Citrus Pectin Research to support your understanding of why this ingredient is so effective in supporting healthy cells and body system.
Acute Kidney Injury Induces Remote Cardiac Damage and Dysfunction Through the Galectin-3 Pathway JACC Basic to Translational Science
Highlights • In 2 different mouse models, AKI increased Gal-3 expression and induced cardiac dysfunction, cardiac and systemic inflammation, cardiac macrophage infiltration, and fibrosis. • Cardiac...
Perindopril and a Galectin-3 Inhibitor Improve Ischemic Heart Failure in Rabbits by Reducing Gal-3 Expression and Myocardial Fibrosis - PubMed
Objective: Ventricular remodeling is considered the basis of heart failure and is involved in myocardial fibrosis. This study aimed to assess perindopril and a galectin-3 inhibitor (modified citrus pectin, MCP) for their effects on ventricular remodeling and myocardial fibrosis in rabbits wit …
Galectin-3 expression and secretion by tumor-associated macrophages in hypoxia promotes breast cancer progression - PubMed
Tumor-associated macrophages (TAMs) have been shown to be associated with poor prognosis of cancer and are predominately localized in the hypoxia regions of tumor. We demonstrated in this study that hypoxia increases the synthesis and secretion of galectin-3 by TAMs. The increased expression of gale …